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Updated: Jun 26, 2026
Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture
Published on: May 14, 2015
[Programmed application of extracellular matrix promotes neural differentiation of mouse embryonic stem cells]
Zhi-yan Shan1, Lei Lei, Ya-jun Chen
1Department of Histology and Embryology, Harbin Medical University, Harbin 150081, China. shanzhiyan@yahoo.com.cn
Objective:
To study the role of extracellular matrix (ECM) in neural differentiation of mouse embryonic stem cells (ESCs).
Methods:
Mouse ESCs were incubated in the ESC conditioned medium, and the formation of embryonic bodies (EBs) were induced in bacteriological dishes using high-concentration all-trans retinoic acid (RA). The EBs were seeded on different matrixes (gelatin, fibronectin, and laminin/poly-L-ornithine) to test their impact on neural differentiation of the ESCs using immunofluorescence assay. The effect of laminin/poly-L-ornithine on the growth of neurites was evaluated with fluorescence microscopy.
Results:
High-concentration RA activated and accelerated the differentiation of ESCs toward nestin-positive neural progenitor cells. Fibronectin supplement in the matrix dose-dependently promoted ESC differentiation into neural progenitor cells, while laminin/poly-L-ornithine increased the growth of the neurites and induced the maturation of the differentiated neural cells.
Conclusion:
ECM plays an important role in neural differentiation of mouse ESCs, and application of FN produces the most conspicuous effect during the differentiation of the ESCs into the neural progenitor cells;laminin/poly-L-ornithine is the most effective during their differentiation into neural cells.
