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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

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Related Experiment Video

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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

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Published on: April 26, 2017

Splicing factor SFRS1 recognizes a functionally diverse landscape of RNA transcripts.

Jeremy R Sanford1, Xin Wang, Matthew Mort

  • 1Department of Molecular, Cellular, and Developmental Biology, University of California Santa Cruz, Santa Cruz, California 95064, USA. sanford@biology.ucsc.edu

Genome Research
|January 1, 2009
PubMed
Summary

The splicing factor SFRS1 binds thousands of RNA transcripts, including mRNA and non-coding RNAs. Mutations disrupting SFRS1 binding sites impact gene expression, revealing its broad regulatory role.

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Area of Science:

  • Molecular Biology
  • Genetics
  • RNA Biology

Background:

  • Metazoan gene expression relies on the genetic code for protein structure and the splicing code for proteomic diversity via alternative splicing.
  • SFRS1 is a crucial splicing factor regulating pre-mRNA splicing.

Purpose of the Study:

  • To define the binding specificity and regulatory roles of the splicing factor SFRS1.
  • To investigate the impact of mutations on SFRS1 binding sites.

Main Methods:

  • Cross-linking immunoprecipitation and high-throughput sequencing (CLIP-seq) to identify SFRS1 binding sites.
  • Bioinformatic analysis of binding site enrichment and motif identification.
  • Search of the Human Gene Mutation Database for mutations affecting SFRS1 binding sites.

Main Results:

  • CLIP-seq identified 23,632 SFRS1 binding sites across various RNA classes (mRNA, miRNA, snoRNA, ncRNA).
  • A purine-rich consensus motif was identified as the canonical SFRS1 binding site, enriched near splice sites.
  • Genes encoding RNA processing factors were overrepresented among SFRS1-bound mRNAs.
  • 181 mutations in 82 genes were found to disrupt predicted SFRS1 binding sites.

Conclusions:

  • SFRS1 plays a broad role in post-transcriptional gene regulation by binding diverse RNA transcripts.
  • Disruption of SFRS1 binding sites by mutations can affect gene expression.
  • This study expands the known functions of SR proteins in RNA regulation.