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Published on: July 14, 2015
Performance of polymeric materials for solid phase extraction prior to chromatographic analysis
Denise Bohrer1, Marlei Veiga dos Santos, Adrian G Ramirez
1Departamento de Química, Universidade Federal de Santa Maria, 97 105-900 Santa Maria, Brazil. ndenise@quimica.ufsm.br
Synthetic polymers like polyethylene and polyurethane effectively clean biological samples for analysis. These materials show promise as adsorbents in solid-phase extraction, outperforming conventional methods for protein and component removal.
Area of Science:
- Analytical Chemistry
- Materials Science
Background:
- Biological sample analysis often requires extensive clean-up to remove interfering components like proteins.
- Conventional adsorbents for solid-phase extraction (SPE) have limitations in efficiency and matrix compatibility.
Purpose of the Study:
- To evaluate synthetic polymeric materials as alternatives to conventional adsorbents for biological sample clean-up.
- To compare the efficiency of various polymers in removing proteins and other matrix components from serum, urine, and tissue extracts.
Main Methods:
- Synthetic polymers (polyethylene, polyurethane) and conventional SPE cartridges were tested for their ability to adsorb biological components.
- Protein content was measured in serum and liver homogenates after treatment.
- Urine samples were analyzed via UV spectroscopy after percolation through polymer columns.
- Cleaned samples were analyzed using C-18 chromatography with UV detection to assess overall clean-up effectiveness.
Main Results:
- High-density polyethylene (HDPE) and ultra-high molecular weight polyethylene (UHMWPE) showed excellent protein retention from serum.
- Polyurethane and polybutadiene (PB) completely retained proteins from liver homogenates.
- Several polymers, including HDPE and UHMWPE, effectively removed interfering substances from urine, resulting in clean UV spectra.
- Conventional SPE cartridges demonstrated varied performance, with limited retention and desorption of biological components.
Conclusions:
- Synthetic polymers, particularly specific types of polyethylene and polyurethane, are effective adsorbents for cleaning biological samples.
- These polymeric materials offer a viable and efficient alternative to conventional adsorbents for solid-phase extraction in biological analysis.
- The study highlights the potential of tailored polymeric materials for improving sample preparation in analytical chemistry.
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