Related Experiment Video
Updated: Jun 26, 2026
Harvesting of Peroneus Longus Tendon Autograft
Published on: September 2, 2025
Tension level during preconditioning influences hamstring tendon graft properties
John J Elias1, Srianjana Kilambi, William J Ciccone
1Medical Education and Research Institute of Colorado, Colorado Springs, Colorado, USA. jelias@agmc.org
Background:
Hamstring tendon grafts used for anterior cruciate ligament reconstruction are preconditioned to reduce the influence of viscoelasticity on the stiffness and tension.
Hypothesis:
Increasing the tension applied during preconditioning will decrease the loss of tension and stiffness due to viscoelasticity.
Study Design:
Controlled laboratory study.
Methods:
Six quadruple-strand hamstring tendon grafts were tested twice in vitro with 2 tension levels applied during preconditioning. The grafts were preconditioned at 80 N or 160 N, followed by 15 minutes of relaxation, and subsequently loaded to 80 N to represent application of initial tension. After 5 minutes of relaxation, the tension was recorded and the stiffness was measured over 0.06 mm of displacement. The tension and stiffness measurements were repeated 3 hours later. The data were compared between the 2 preconditioning levels with a 2-way repeated-measures analysis of variance.
Results:
Graft tension and stiffness were significantly larger (P < .05) for 160 N during preconditioning. For 80 N, the average tension at 5 minutes and 3 hours was 40 N and 21 N, respectively, compared with 50 N and 30 N, respectively, for 160 N. For 80 N, the average stiffness at 5 minutes and 3 hours was 152 N/mm and 124 N/mm, respectively, compared with 173 N/mm and 146 N/mm, respectively, for 160 N.
Conclusion:
Increasing the tension applied to hamstring tendon grafts during preconditioning can decrease the postoperative loss of tension and stiffness due to viscoelasticity.
Clinical Relevance:
The increased graft tension and stiffness could reduce postoperative knee laxity.
