Related Experiment Video
Updated: Jun 26, 2026

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
Published on: February 19, 2019
hag expression in Bacillus subtilis is both negatively and positively regulated by ScoC
Prashant Kodgire1, K Krishnamurthy Rao1
1School of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
Abstract:
In Bacillus subtilis, motility and chemotaxis require the expression of hag, which encodes flagellin. This gene is transcribed by the sigma(D) form of RNA polymerase and is regulated by a group of proteins called transition state regulators (TSRs). Our studies show that hag transcription is negatively regulated by the transition state regulator ScoC, by binding to its promoter. Furthermore, ScoC, indirectly, also positively regulates hag by increasing the availability of sigma(D) by downregulating the levels of the anti-sigma(D)-factor FlgM. We further show that the positive regulation by ScoC predominates over the negative regulation.
Related Concept Videos
Regulation of Bacterial Virulence
Gene Regulation During Sporulation
Gene Regulation in Microbial Communities: Quorum Sensing
Stringent Response in E. coli
Global Regulatory Systems
Inducible Operons: lac Operon

