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Updated: Jun 26, 2026

Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria
Published on: September 16, 2013
Sero-characterization of lipopolysaccharide from Burkholderia thailandensis
Omar Qazi1, Joann L Prior, Barbara M Judy
1Department of Pediatrics and the Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX 77555, USA.
Abstract:
We report the successful purification of lipopolysaccharide (LPS) from Burkholderia thailandensis, a Gram-negative bacterium, closely related to the highly pathogenic organisms B. pseudomallei and B. mallei. Burkholderia thailandensis LPS is shown to cross-react with rabbit and mouse sera obtained from inoculation with B. pseudomallei or B. mallei, respectively. These data suggest that B. thailandensis LPS shares similar structural features with LPS molecules from highly pathogenic Burkholderia species. This information may prove useful in ongoing efforts to develop novel vaccines and/or diagnostic reagents.
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