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Concerted effects of two activator modules on the group I ribozyme reaction
Yoshiya Ikawa1, Tomoaki Shiohara, Shoji Ohuchi
1Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, Fukuoka 819-0351, Japan.
Abstract:
Group I intron ribozymes have a modular architecture and structural elements essential for catalysis. The elements are located in the conserved modular domain P3-P7 that is stabilized by another conserved module, P4-P6. It has been reported that artificial modules can complement the function of the native P4-P6. To exploit the modular architecture of group I ribozyme, we have constructed a hybrid ribozyme by attaching an artificial activator module to the wild-type T4 td ribozyme. Kinetic analysis of the hybrid ribozyme revealed that the artificial module and P4-P6 have unusual positive and negative concerted effects in activating the ribozyme.
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