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Updated: Jun 26, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
Synthesis, characterization and DNA-binding properties of the Cu(II) complex with
Gao-Fei Qi1, Zheng-Yin Yang, Dong-Dong Qin
1College of Chemistry and Chemical Engineering and State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou , China.
Abstract:
A new chromone derivative (7-methoxychromone-3-carbaldehyde-benzoylhydrazone) ligand (HL) and its novel Cu(II) complex have been synthesized and characterized on the basis of elemental analyses, molar conductivities, proton nuclear magnetic resonance, infrared spectra and mass spectra analyses. The general formula of the Cu(II) complex is [CuL(HB2BO)]Cl x 2H2O. In addition, the interaction of the Cu(II) complex and its free ligand with calf-thymus DNA was investigated by electronic absorption spectroscopy, fluorescence spectroscopy and viscosity measurement. Results suggest that both the two compounds can bind with calf-thymus DNA via an intercalation mechanism. Furthermore, the Cu(II) complex can bind to DNA more strongly than the free ligand due to the chelating effect of copper(II) ion to the free ligand.
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