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Updated: Jun 26, 2026
Viral Tracing of Genetically Defined Neural Circuitry
Published on: October 17, 2012
Genetically timed, activity-sensor and rainbow transsynaptic viral tools
Zsolt Boldogkoi1, Kamill Balint, Gautam B Awatramani
1Department of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., Szeged, H-6720, Hungary.
Abstract:
We developed retrograde, transsynaptic pseudorabies viruses (PRVs) with genetically encoded activity sensors that optically report the activity of connected neurons among spatially intermingled neurons in the brain. Next we engineered PRVs to express two differentially colored fluorescent proteins in a time-shifted manner to define a time period early after infection to investigate neural activity. Finally we used multiple-colored PRVs to differentiate and dissect the complex architecture of brain regions.
