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Updated: Jun 26, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
Published on: October 2, 2021
[Compensation of backscattering coefficient in Fourier-domain optical coherence tomography]
Ling Lin1, Tae-Sok Zhang, Zhao Ren
1State Key Laboratory of Precision Measurement Technology and Instruments, Tianjin University, Tianjin 300072, China.
Abstract:
According to the phenomenon that the intensity of backscattering light decreases dramatically as the detecting depth increases, causing blurred imaging in Fourier-domain optical coherence tomography (FDOCT), a compensation method used for different detecting depth was advanced in the present paper. Through compensating the incident power and scattering coefficient of back-scattered light in each layer of the sample, the real gray level of the image was obtained. With the model of multi-layered homogeneous tissue, the main factors influencing image gray were analyzed. Establishing the FDOCT system with multi-layer cover-glass to be the sample, the structure image was obtained. On the basis of cover-glass as the sample, the general formulas for multi-layer compensation were derived. Combining the theoretical compensation and experimental situation, the obtained data were compensated from incident and emergent intensity. The results show that this compensation method is an effective and feasible way to make the deep layer more visible.
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