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Updated: Jun 26, 2026

Rapid Setup of Tissue Microarray and Tiled Area Imaging on the Multiplexed Ion Beam Imaging Microscope Using the Tile/SED/Array Interface
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Rapid Setup of Tissue Microarray and Tiled Area Imaging on the Multiplexed Ion Beam Imaging Microscope Using the Tile/SED/Array Interface

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Microbeam-integrated multiphoton imaging system.

Alan W Bigelow1, Charles R Geard, Gerhard Randers-Pehrson

  • 1Center for Radiological Research, Columbia University, New York, New York 10032, USA.

The Review of Scientific Instruments
|January 7, 2009
PubMed
Summary
This summary is machine-generated.

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Multiphoton microscopy enhances radiation research by enabling 3D imaging of cellular dynamics in tissuelike samples after irradiation. This advanced technique improves observation of postirradiation effects in biological studies.

Area of Science:

  • Radiological research
  • Cellular dynamics
  • Advanced imaging techniques

Background:

  • The Radiological Research Accelerator Facility (RARAF) at Columbia University utilizes the Microbeam II cell irradiator for radiation effect studies.
  • Existing imaging techniques were enhanced to observe cellular responses to radiation in real-time.

Purpose of the Study:

  • To integrate multiphoton microscopy into the existing radiation research setup at RARAF.
  • To enable high-resolution, three-dimensional (3D) imaging of cellular dynamics postirradiation.

Main Methods:

  • Custom-designed and integrated a multiphoton microscope into a Nikon Eclipse E600-FN fluorescence microscope.
  • Utilized multiphoton excitation for laser-scanning, 3D imaging capabilities.
  • Applied the technique to tissuelike samples, including small living organisms.

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Last Updated: Jun 26, 2026

Rapid Setup of Tissue Microarray and Tiled Area Imaging on the Multiplexed Ion Beam Imaging Microscope Using the Tile/SED/Array Interface
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Published on: September 15, 2023

Fluorescence Lifetime Macro Imager for Biomedical Applications
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Main Results:

  • Successfully incorporated a 3D multiphoton microscope into the Microbeam II cell irradiator endstation.
  • Established an enhanced imaging routine for radiation experiments.
  • Demonstrated the capability to observe postirradiation cellular dynamics in biological samples.

Conclusions:

  • Multiphoton microscopy provides a powerful tool for studying radiation effects at the cellular level.
  • The integrated system offers advanced 3D imaging for biological applications in radiation research.
  • This enhancement at RARAF facilitates deeper insights into radiation biology.