Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Mitochondrial complex II in intestinal epithelial cells regulates T cell-mediated immunopathology.

Nature immunology·2026
Same author

On-Scanner Correction of Gradient Nonlinearity Bias for Accurate Assessment of Diffusion Heterogeneity Across Bone Sites in Myelofibrosis Patients.

Magnetic resonance in medicine·2026
Same author

Quantitative MRI Assessment of Bone Marrow Disease in Myelofibrosis: A Prospective Study.

Radiology. Imaging cancer·2025
Same author

Murine Functional Lung Imaging Using X-Ray Velocimetry for Longitudinal Noninvasive Quantitative Spatial Assessment of Pulmonary Airflow.

Tomography (Ann Arbor, Mich.)·2025
Same author

Fat Fraction MRI for Longitudinal Assessment of Bone Marrow Heterogeneity in a Mouse Model of Myelofibrosis.

Tomography (Ann Arbor, Mich.)·2025
Same author

A deep learning aided bone marrow segmentation of quantitative fat MRI for myelofibrosis patients.

Frontiers in oncology·2025

Related Experiment Video

Updated: Jun 26, 2026

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
10:04

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models

Published on: March 13, 2018

Enhancing Akt imaging through targeted reporter expression.

Limin Zhang1, Mahaveer S Bhojani, Brian D Ross

  • 1Department of Radiation Oncology, Center for Molecular Imaging, University of Michigan, Ann Arbor, MI 48109, USA.

Molecular Imaging
|January 7, 2009
PubMed
Summary

A novel membrane-bound bioluminescent Akt reporter (MyrPalm-BAR) offers a sensitive and quantitative method for monitoring Akt kinase activity. This tool aids in discovering new cancer drugs targeting the Akt pathway.

More Related Videos

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain
09:59

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

Published on: March 31, 2022

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
09:22

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Published on: November 26, 2013

Related Experiment Videos

Last Updated: Jun 26, 2026

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
10:04

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models

Published on: March 13, 2018

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain
09:59

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

Published on: March 31, 2022

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
09:22

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Published on: November 26, 2013

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Biotechnology

Background:

  • The serine/threonine kinase Akt (Protein Kinase B) is crucial for cancer cell survival and therapy resistance.
  • Targeting Akt is a significant strategy in cancer treatment.
  • Molecular imaging aids in drug discovery, efficacy assessment, and target validation.

Purpose of the Study:

  • To develop a genetically engineered bioluminescent reporter for Akt kinase activity.
  • To create a membrane-localized version (MyrPalm-BAR) for enhanced sensitivity and quantification of Akt activity.
  • To validate the reporter's utility in monitoring Akt pathway modulation by small molecule inhibitors.

Main Methods:

  • Construction of a hybrid bioluminescent Akt reporter (BAR) molecule.
  • Modification to create a membrane-bound version (myristoylated and palmitoylated bioluminescent Akt reporter [MyrPalm-BAR]).
  • Utilized bioluminescence imaging to monitor Akt activity in response to small molecule inhibitors targeting Akt and upstream kinases (EGFR, PI3K).

Main Results:

  • The membrane-targeted MyrPalm-BAR reporter demonstrated higher sensitivity and quantitative accuracy compared to non-membrane-bound reporters.
  • Bioluminescence imaging effectively monitored changes in Akt activity upon inhibition of Akt itself or upstream signaling pathways.
  • The reporter quantitatively tracked Akt activation status changes induced by small molecule inhibitors.

Conclusions:

  • The membrane-associated Akt reporter (MyrPalm-BAR) is a sensitive and quantitative tool for assessing Akt kinase activity.
  • This reporter is well-suited for high-throughput screening to identify novel compounds that modulate the Akt pathway.
  • The MyrPalm-BAR reporter facilitates drug discovery and validation in cancer therapy research.