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Development and validation of a LC/MS/MS method for quantification of nobiliside A in rat plasma
Dan Guo1, Yang Xiong, Yue Zhang
1Department of Pharmaceutical Science, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Abstract:
A LC/MS/MS method was developed and validated for determination of nobiliside A in rat plasma. Analyses were separated by an Agella Venusil ASB-C18 column and isocratic elution with methanol:water (80:20, v/v) as a mobile phase. A tandem mass spectrometer was used as a detector for quantitative analysis. Calibration curves (R(2)>0.999) in spiked plasma were linear over the concentration range of 50-5000 ng/mL. The overall accuracy of this method was 93-104% for nobiliside A. Within-day and between-day variability was both less than 9% in plasma. The data indicate that the LC/MS/MS method is an effective method for the pharmacokinetics study of nobiliside A in rat plasma.
