Peripheral venous occlusion causing cardiac hypertrophy and changes in biological parameters in rats
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1Department of Human and Engineered Environmental Studies, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba 277-8563, Japan. kawada@k.u-tokyo.ac.jp
Insights
Peripheral vascular disease (PVD) alone can cause cardiac hypertrophy. This study found PVD-induced changes in growth factors and heat shock proteins (HSPs) in heart muscle, impacting cardiac function.
Area of Science:
- Cardiovascular Biology
- Molecular Cardiology
- Vascular Medicine
Background:
- Peripheral vascular disease (PVD) is common in patients with coronary artery disease and myocardial hypertrophy.
- The direct impact of PVD on cardiac hypertrophy and its underlying subcellular mechanisms are not fully understood.
Purpose of the Study:
- To investigate the effects of PVD alone on cardiac muscle.
- To identify changes in biological parameters influencing cardiac hypertrophy and cardioprotection in the context of PVD.
Main Methods:
- Peripheral vascular disease was induced in rats via hind limb vein occlusion.
- Cardiac hypertrophy was assessed by measuring heart weight relative to body weight.
- Expression levels of insulin-like growth factor-1, myostatin, and mechano growth factor were analyzed.
- Tissue concentrations of nitric oxide and heat shock proteins (HSP-72, HSP-90, heat shock cognate protein-70) were measured.
Main Results:
- A significant increase in heart weight relative to body weight (approximately 10%) was observed in the PVD group compared to controls.
- Insulin-like growth factor-1 expression increased, while myostatin and mechano growth factor decreased in the PVD group.
- Tissue nitric oxide concentration and levels of HSP-72, HSP-90, and heat shock cognate protein-70 were significantly reduced in the hypertrophied myocardium.
Conclusions:
- Peripheral vascular occlusion alone is sufficient to induce cardiac hypertrophy.
- PVD is associated with alterations in key biological parameters, including growth factors and heat shock proteins, within the myocardium.
- These molecular changes may contribute to the development of cardiac hypertrophy in PVD patients.
Abstract:
Although patients with peripheral vascular disease (PVD) show a high prevalence of coronary artery disease and myocardial hypertrophy, influences of PVD alone on cardiac hypertrophy, and its subcellular mechanism remain unclear. This study investigates the influences of PVD alone on cardiac muscles and the changes in biological parameters that potentially affect cardiac hypertrophy and cardioprotection. Several veins from rat hind limb were occluded. Two weeks later, the weight of heart relative to body weight in the occluded group increased by approximately 10% compared to that in the sham-operated group. A significant increase was observed in insulin-like growth factor-1 expression, and a significant decrease was observed in myostatin and mechano growth factor in the occluded group compared to the sham-operated group. Tissue concentration of nitric oxide, and content of heat shock protein (HSP)-72, HSP-90, and heat shock cognate protein-70 significantly decreased in the hypertrophied myocardium. These results suggest that peripheral occlusion alone causes cardiac hypertrophy associated with changes in biological parameters.
