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Updated: Jun 26, 2026
Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
Published on: March 8, 2012
[Application of Epstein-Barr virus-transformed B lymphoblastic cells in identification of CTL epitopes specific for
Mei-Liang Wang1, Quan-Hua Zhang, Jiu-Ping Wang
1Anning Branch of Lanzhou General Hospital, Lanzhou Military Command, China.
Aim:
To establish Epstein-Barr virus (EBV)-transformed B lymphoblastic cell lines (B-LCL) to present peptides as antigen-presenting cells (APC) and stimulate short-cultured T cells secreting IFN-gamma, by which the T cell epitopes are identified.
Methods:
PBMCs from patients with hemorrhagic fever with renal syndrome (HFRS) were transformed using EBV from supernatant of B95-8 cells. ELISPOT assay was then employed to evaluate the IFN-gamma production of short-cultured G9L-specific CD8(+) T cells stimulated with peptide-pulsed autologous B-LCL cells.
Results:
B-LCL pulsed with G9L or G9L-nested V15R can stimulate G9L-specific CD8(+) T cells producing IFN-gamma, but not B-LCL pulsed with non-homologous I15P.
Conclusion:
B-LCL can efficiently and specifically present peptides to peptide-specific T cells as non-professional APC.
