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Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

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Related Experiment Video

Updated: Jun 26, 2026

Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice

Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice

Published on: January 11, 2017

RNA interference screening demystified.

C J Lord¹, S A Martin, A Ashworth

¹The Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, London, UK. Chris.Lord@icr.ac.uk

Journal of Clinical Pathology

|January 8, 2009

Summary

This summary is machine-generated.

Genetic screens using RNA interference (RNAi) systematically assess gene function impacts on cell behavior. This review details RNAi screening methods and their relevance for pathologists studying disease.

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Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice

Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice

Published on: January 11, 2017

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

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Published on: May 23, 2025

Area of Science:

Molecular Biology
Genetics
Cell Biology

Background:

Genetic screens systematically evaluate gene function's impact on cellular behavior.
RNA interference (RNAi) technology enables large-scale genetic screening.
Understanding gene function is crucial for disease pathogenesis and treatment.

Purpose of the Study:

To explain the various formats of RNA interference (RNAi) screening.
To describe the applications of RNAi screening relevant to research pathology.
To highlight the utility of RNAi screens in understanding disease.

Main Methods:

Systematic assessment of gene function modification.
Application of RNA interference (RNAi) technology.
Analysis of cell behavior in response to genetic alterations.

Main Results:

RNAi screening is becoming a standard method in genetic research.
Different formats of RNAi screens exist, each with specific advantages.
RNAi screening provides valuable data for disease research.

Conclusions:

RNAi screening is a powerful tool for dissecting gene function.
The applications of RNAi screening are highly relevant to research pathologists.
This technique aids in understanding disease mechanisms and identifying therapeutic targets.