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Effect of disodium monofluorphosphate on plasma and blood viscosity in the rat
V Di Loreto1, A Rigalli, L Cinara
1Bone Biology and Mineral Metabolism Laboratory, School of Medicine, Rosario National University, Argentina.
Abstract:
Alpha macroglobulins (AM) are plasma proteins whose main function is to inactivate proteinases, protecting the tissues from the action of these enzymes. AM have influence on plasma viscosity (PV) and binds monofluorophosphate (MFP), which disturbs its homeostasis. The aim of this work was to evaluate whether the administration with MFP could modify blood viscosity. AM levels (micromol/l), PV (mPa.s), viscosity of red blood cells suspensions in NaCl 9 g/l (VES) and in autologue plasma (VEP) were measured in fifty-day old rats after a single dose of 80 micromol MFP or after 30 days of treatment with 80 micromol of MFP. Relative viscosity (RV) was calculated as the ratio VEP/PV. AM and PV increased significantly after 30 min of an oral dose of MFP. Controls (n=6), AM: 19.65+/-0.85, PV: 1.39+/-0.01, treated (n=6), AM: 22.88+/-0.75 (p<0.05), PV: 1.76+/-0.14 (p<0.05). After 30 days of treatment with MFP, AM and PV increased significantly. Controls (n=6), AM: 10.76+/-1.33, PV: 1.19+/-0.04, treated (n=6), AM: 17.66+/-1.27 (p<0.05), PV: 1.38+/-0.03 (p<0.05). The treatment with MFP modifies neither the VEP nor the RV. These results would indicate that AM and/or MFP did not interact with erythrocyte membrane and did not modify erythrocyte deformability.
Insights
Monofluorophosphate (MFP) administration increased alpha macroglobulins (AM) and plasma viscosity (PV) in rats. However, MFP did not alter red blood cell viscosity or relative viscosity, suggesting no impact on erythrocyte deformability.
Area of Science:
- Biochemistry
- Physiology
- Hematology
Background:
- Alpha macroglobulins (AM) are key plasma proteins that regulate proteinase activity, protecting tissues.
- AM influence plasma viscosity (PV) and interact with monofluorophosphate (MFP), potentially disrupting homeostasis.
- Understanding MFP's effect on blood viscosity is crucial for its physiological implications.
Purpose of the Study:
- To investigate the impact of monofluorophosphate (MFP) administration on blood viscosity parameters in rats.
- To evaluate changes in alpha macroglobulins (AM) and plasma viscosity (PV) following MFP exposure.
- To determine if MFP affects red blood cell suspension viscosity (VES) and relative viscosity (RV).
Main Methods:
- Rats were administered a single dose or treated for 30 days with monofluorophosphate (MFP).
- Measurements included alpha macroglobulins (AM) levels, plasma viscosity (PV), red blood cell suspension viscosity (VES), and viscosity in autologous plasma (VEP).
- Relative viscosity (RV) was calculated as VEP/PV.
Main Results:
- A single dose of MFP significantly increased AM levels and PV within 30 minutes.
- Chronic 30-day MFP treatment also led to significant increases in AM and PV.
- Neither VES nor RV was significantly altered by MFP treatment, indicating no change in erythrocyte deformability.
Conclusions:
- Monofluorophosphate (MFP) administration demonstrably elevates alpha macroglobulins (AM) and plasma viscosity (PV) in rats.
- MFP does not appear to interact with the erythrocyte membrane or affect erythrocyte deformability.
- The study suggests that while MFP impacts plasma components, its effect on red blood cell function is minimal.
