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Related Experiment Video

Updated: Jun 26, 2026

Identifying Protein-protein Interaction in Drosophila Adult Heads by Tandem Affinity Purification (TAP)

Identifying Protein-protein Interaction in Drosophila Adult Heads by Tandem Affinity Purification (TAP)

Published on: December 5, 2013

[Dicer interacts with Wig-1 protein].

Jian Huang¹, Hai Bo Wang, Ling Yao

¹Department of Biochemistry and Molecular Biology, Shanghai Jiaotong University, School of Medicine, Shanghai 200025. hj1970@hotmail.com

Fen Zi Xi Bao Sheng Wu Xue Bao = Journal of Molecular Cell Biology

|January 9, 2009

Summary

This summary is machine-generated.

This study shows that Dicer, an enzyme crucial for RNA interference, directly interacts with Wig-1, a protein involved in dsRNA binding. Wig-1 enhances Dicer

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Area of Science:

Molecular Biology
Gene Regulation

Background:

Dicer is a key enzyme in RNA interference (RNAi) with double-stranded RNAse (dsRNase) activity.
Wig-1 is a p53-induced protein possessing dsRNA-binding capabilities.

Purpose of the Study:

To investigate the potential interaction between Dicer and Wig-1.
To determine if Wig-1 influences the enzymatic activity of Dicer.

Main Methods:

GST pull-down assays to assess Dicer-Wig-1 association.
Far-western blot analysis to confirm direct binding.
In vitro assays to measure Dicer's dsRNase activity in the presence of Wig-1.

Main Results:

Demonstrated a direct, dose-dependent interaction between Dicer and Wig-1.
Showed that Wig-1 can compete with eIF2C1 for Dicer binding.
Confirmed that Wig-1 enhances the dsRNase activity of Dicer in vitro.

Conclusions:

Dicer and Wig-1 directly interact, suggesting a functional relationship.
Wig-1's ability to enhance Dicer activity implies a role in dsRNA-related gene regulation.