Related Experiment Video
Updated: Jun 26, 2026

Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
Published on: January 22, 2014
Protein engineering, expression, and activity of a novel fusion protein possessing keratinocyte growth factor 2 and
1Department of Biochemistry, Inha University School of Medicine, Incheon, Korea.
Abstract:
Growth factor-induced proliferation and differentiation often require adhesion of cells to the extracellular matrix proteins such as fibronectin (FN). In this study, we aimed to investigate the effect of protein engineering of the keratinocyte growth factor 2 (KGF2) fused to the FN on the mitogenic activity of KGF2. The fusion protein (KGF2-FN10), which was expressed in Escherichia coli, showed significantly enhanced mitogenic activity of KGF2 on human keratinocytes. Moreover, KGF2-FN10 fusion protein showed significantly increased activity to differentiate keratinocytes from native KGF2. In conclusion, these results suggest that KGF2-FN10 fusion protein has certain advantages over native KGF2 and may offer a novel strategy to potentiate the therapeutic effect of KGF2.
Related Concept Videos
Tagging and Fusion Proteins
Fibronectins Connect Cells with ECM
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
Protein Complexes with Interchangeable Parts
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Matrix Proteoglycans and Glycoproteins
