Related Concept Videos
01:15Western Blotting
Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
01:33Enzyme-Linked Immunosorbent Assay
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
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09:32
Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
Published on: September 10, 2017
Point-of-care testing of proteins.
1iPOC Research Group, University of Potsdam, Institute of Biochemistry and Biology, Building 25, Karl-Liebknecht-Str. 24-25, 14476, Golm, Germany. warsinke@uni-potsdam.de
Analytical and Bioanalytical Chemistry
|January 9, 2009
Summary
Point-of-care testing (POCT) offers rapid diagnostics at patient sites. This review explores current POCT protein methods and future electrochemical immunosensor technologies for improved diagnostics.
Area of Science:
- Clinical diagnostics and in vitro diagnostics market analysis.
Background:
- Point-of-care testing (POCT) is a rapidly advancing field in clinical diagnostics.
- POCT involves decentralized testing performed at the patient's location.
- Handheld blood glucose sensors are prominent POCT devices, offering rapid results from small blood samples.
Purpose of the Study:
- To review current methods for protein determination using POCT.
- To discuss emerging technologies, such as electrochemical immunosensors, for future POCT of proteins.
Main Methods:
- Analysis of current POCT devices for protein determination, primarily focusing on lateral flow technology.
- Exploration of advanced techniques with potential for future POCT applications.
Main Results:
- Current lateral flow devices for protein analysis are convenient but often provide only qualitative or semi-quantitative results.
- Emerging technologies show promise for more quantitative and sensitive protein POCT.
Conclusions:
- POCT is a key driver for the future in vitro diagnostics market.
- Electrochemical immunosensors represent a significant advancement for future protein POCT, potentially overcoming limitations of current methods.