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Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...

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In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function
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Differential requirement for CARMA1 in agonist-selected T-cell development.

Benjamin D Medoff1, Barry P Sandall, Aimee Landry

  • 1Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02114, USA. bmedoff@partners.org

European Journal of Immunology
|January 9, 2009
PubMed
Summary

Caspase recruitment domain-containing membrane-associated guanylate kinase protein-1 (CARMA1) is crucial for T cell development. CARMA1 deficiency impairs regulatory T cell (Treg) development but not natural killer T (NKT) cells, revealing its differential role in T cell signaling.

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Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy
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Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy
06:51

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Published on: December 17, 2019

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • TCR signaling is vital for T cell activation and development of specific T cell subsets.
  • CARMA1 is a key mediator in TCR-induced NF-kappaB signaling.
  • The role of CARMA1 in agonist-selected T cell lineage development remains largely unknown.

Purpose of the Study:

  • To investigate the role of CARMA1 in the development of agonist-selected T cell subsets.
  • To elucidate the differential functions of CARMA1 in T cell lineage specification.

Main Methods:

  • Analysis of CARMA1-deficient (CARMA1(-/-)) mice.
  • Flow cytometry to assess T cell populations.
  • TCR signaling pathway analysis.

Main Results:

  • CARMA1(-/-) mice exhibit impaired natural and adaptive Treg development.
  • NKT cell populations are normal in CARMA1(-/-) mice.
  • CD8-alpha alpha T cells are enriched in the gut, and memory-phenotype CD4(+) T cells are reduced in CARMA1(-/-) mice.

Conclusions:

  • CARMA1 is essential for Treg development.
  • CARMA1 is not required for NKT cell development.
  • CARMA1-mediated TCR signaling plays a differential role in the development of various T cell subsets.