Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 26, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Simple, fast and high-throughput single-cell analysis on PDMS microfluidic chips.

Linfen Yu1, Huaiqing Huang, Xiuling Dong

  • 1Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, PR China.

Electrophoresis
|January 9, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhanced stability of immobilized xylanase with nano‑zinc oxide/ethyl cellulose composite carrier for efficient xylan hydrolysis.

Food chemistry·2026
Same author

Polybenzimidazole-based magnetic solid-phase extraction coupled with gas chromatography-mass spectrometry for the determination of triazine herbicides in environmental waters.

Journal of chromatography. A·2026
Same author

Repurposing the Antibiotic Tigecycline to Inhibit Tumor Growth and Hormone Secretion in Somatotroph Pituitary Neuroendocrine Tumors.

International journal of endocrinology·2026
Same author

Regulating B-configuration in N-doped carbon to enhance H<sub>2</sub>O<sub>2</sub> electrosynthesis and Fe<sup>3+</sup>/Fe<sup>2+</sup> cycling for electro-Fenton water purification.

Journal of environmental management·2026
Same author

Cholangioscopy-guided diagnosis and management of biliary cast syndrome in a nontransplant patient.

VideoGIE : an official video journal of the American Society for Gastrointestinal Endoscopy·2026
Same author

The overlooked fate of piperazine contaminants containing imino groups in the protocatechuic acid/periodate process: Role of ortho-benzoquinones.

Water research·2026
Same journal

Kinship Inferences for Second-Degree Relatives With a Combination of STRs and Microhaplotypes.

Electrophoresis·2026
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
See all related articles

This study presents a fast, high-throughput microfluidic chip for single-cell chemical analysis. The method rapidly lyses and detects molecules in individual cells, enabling efficient study of cellular processes.

Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Microfluidics

Background:

  • Single-cell analysis is crucial for understanding cellular heterogeneity.
  • Existing methods can be slow and low-throughput.
  • Microfluidic platforms offer potential for rapid, high-throughput analysis.

Purpose of the Study:

  • To develop a simple, fast, and high-throughput microfluidic chip for chemical analysis of single cells.
  • To demonstrate the capability of the chip for analyzing intracellular molecules.

Main Methods:

  • Utilized a cross polydimethylsiloxane (PDMS) microfluidic chip.
  • Employed hydrodynamic force for sequential cell loading.
  • Applied 0.2% sodium dodecyl sulfate (SDS) for cell lysis.
  • Detected cellular lysates using Laser-Induced Fluorescence (LIF).

More Related Videos

A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation
10:24

Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation

Published on: September 19, 2019

Related Experiment Videos

Last Updated: Jun 26, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation
10:24

Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation

Published on: September 19, 2019

Main Results:

  • Achieved cell lysis within 500 ms per cell.
  • Demonstrated reliable analysis of glutathione and rhodamine 123 in single K562 cells.
  • Analyzed approximately 100 cells in about 10 minutes, indicating high throughput.

Conclusions:

  • The developed microfluidic method is simple, fast, and high-throughput.
  • This approach is significant for identifying molecules in fast biochemical processes.
  • The method is valuable for studying heterogeneous cell populations.