Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved in...
Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule01:10

Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule

In the AX proton spin system, proton A can sense the two spin states of a coupled proton X, resulting in a doublet NMR signal with two peaks of equal (1:1) intensity. When proton A is coupled to two equivalent protons (AX2 spin system), the spin states of each X can be aligned with or against the external field, creating three possible scenarios. This results in a 1:2:1  triplet signal, where the central peak corresponds to the chemical shift of A and is twice as large or intense as the others.
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Response to: 'Long term night shift work, genetic predisposition, and risk of incident asthma: a prospective study'.

QJM : monthly journal of the Association of Physicians·2024
Same author

Long-term night shift work, genetic predisposition and risk of incident asthma: a prospective cohort study.

QJM : monthly journal of the Association of Physicians·2024
Same author

Preoperative BMI and Hb levels are important predictors of massive bleeding in liver transplant patients.

European review for medical and pharmacological sciences·2024
Same author

Mass spectrometry-based metabolic profiling for identification of biomarkers related to footpad dermatitis in ducks.

British poultry science·2023
Same author

Efficacy of clonidine in the treatment of children with tic disorder co-morbid with attention deficit hyperactivity disorder.

European review for medical and pharmacological sciences·2023
Same author

CircOMA1 modulates cabergoline resistance by downregulating ferroptosis in prolactinoma.

Journal of endocrinological investigation·2023

Related Experiment Video

Updated: Jun 26, 2026

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
12:47

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins

Published on: December 27, 2016

Correlation NMR spectroscopy involving quadrupolar nuclei.

J P Amoureux1, J Trébosc, L Delevoye

  • 1UCCS, CNRS-8181, Lille-University, Villeneuve d'Ascq, Fr-59652, France. Jean-paul.amoureux@univ-lille1.fr

Solid State Nuclear Magnetic Resonance
|January 10, 2009
PubMed
Summary

Recent advancements enable high-resolution observation and analysis of integer or half-integer quadrupolar nuclei. This review covers methods for studying their through-space and through-bond connectivities.

More Related Videos

Line Shape Analysis of Dynamic NMR Spectra for Characterizing Coordination Sphere Rearrangements at a Chiral Rhenium Polyhydride Complex
10:52

Line Shape Analysis of Dynamic NMR Spectra for Characterizing Coordination Sphere Rearrangements at a Chiral Rhenium Polyhydride Complex

Published on: July 27, 2022

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
14:44

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

Related Experiment Videos

Last Updated: Jun 26, 2026

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
12:47

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins

Published on: December 27, 2016

Line Shape Analysis of Dynamic NMR Spectra for Characterizing Coordination Sphere Rearrangements at a Chiral Rhenium Polyhydride Complex
10:52

Line Shape Analysis of Dynamic NMR Spectra for Characterizing Coordination Sphere Rearrangements at a Chiral Rhenium Polyhydride Complex

Published on: July 27, 2022

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
14:44

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Solid-State NMR

Background:

  • Quadrupolar nuclei, particularly those with integer or half-integer spin values, present unique challenges in NMR spectroscopy due to their complex interactions.
  • Traditional NMR methods often struggle to provide high-resolution data for these nuclei, limiting detailed structural and dynamic investigations.

Purpose of the Study:

  • To provide a comprehensive overview of recent methodological developments for observing and analyzing integer or half-integer quadrupolar nuclei.
  • To highlight techniques that facilitate the study of through-space and through-bond connectivities for these challenging nuclei.

Main Methods:

  • Review of advanced NMR pulse sequences and experimental designs tailored for quadrupolar nuclei.
  • Discussion of solid-state NMR techniques, including magic-angle spinning (MAS) and dynamic-angle spinning (DAS).
  • Analysis of methods for correlating spectral information to determine through-space and through-bond interactions.

Main Results:

  • Recent developments have significantly improved the resolution and sensitivity of NMR spectra for quadrupolar nuclei.
  • Novel techniques allow for the detailed mapping of through-space (e.g., Nuclear Overhauser Effect) and through-bond (e.g., scalar couplings) connectivities.
  • These advancements enable more precise structural elucidation and the study of molecular dynamics.

Conclusions:

  • The reviewed methods offer powerful tools for investigating the structure and dynamics of systems containing integer or half-integer quadrupolar nuclei.
  • Further development in these areas promises to expand the applicability of NMR spectroscopy to a wider range of chemical and biological systems.