Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Role of Matrix Metalloproteases in Degradation of ECM

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...

Masking and Demasking Agents

Masking and Demasking Agents

EDTA titrations may necessitate masking and demasking agents to temporarily protect a particular metal ion in a mixture from the EDTA reaction. These agents facilitate the sequential analysis of the metal ions by forming stable complexes with some—but not all—metal ions during certain steps.
There are many masking agents, such as cyanide, fluoride, triethanolamine, thiourea, and 2,3-bis(sulfanyl)propan-1-ol (formerly 2,3-dimercapto-1-propanol), with the masking agent chosen based on the metal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Entropy Spectroscopy of a Bilayer Graphene Quantum Dot.

Physical review letters·2025

Same author

Search for Lepton-Flavor Violation in Z-Boson Decays with τ Leptons with the ATLAS Detector.

Physical review letters·2022

Same author

Search for New Phenomena in Final States with Two Leptons and One or No b-Tagged Jets at sqrt[s]=13 TeV Using the ATLAS Detector.

Physical review letters·2021

Same author

Search for Displaced Leptons in sqrt[s]=13 TeV pp Collisions with the ATLAS Detector.

Physical review letters·2021

Same author

Longitudinal Flow Decorrelations in Xe+Xe Collisions at sqrt[s_{NN}]=5.44 TeV with the ATLAS Detector.

Physical review letters·2021

Same author

Search for Dark Matter Produced in Association with a Dark Higgs Boson Decaying into W^{±}W^{∓} or ZZ in Fully Hadronic Final States from sqrt[s]=13 TeV pp Collisions Recorded with the ATLAS Detector.

Physical review letters·2021

Same journal

Gold Nanoparticles Enhance the Antibacterial and Osteogenic Properties of Polyetheretherketone.

Journal of dental research·2026

Same journal

Periodontitis-Aggravated Diabetic Kidney Disease with Altered Glycolysis.

Journal of dental research·2026

Same journal

Response to Letter to Editor: "Estimating the Individualized Effect of Tooth Extraction before Radiotherapy on Osteoradionecrosis Using Causal Machine Learning".

Journal of dental research·2026

Same journal

Reorienting Oral Health Promotion through Systems Thinking.

Journal of dental research·2026

Same journal

<i>Porphyromonas gingivalis</i>-Induced NETs Mediate Neuroinflammation via TLR4 Activation.

Journal of dental research·2026

Same journal

Oral Burden of Sjögren Disease: A Systematic Review and Meta-analysis.

Journal of dental research·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 26, 2026

Establishment of a Murine Pulp Exposure Model with a Novel Mouth-Gag for Pulpitis Research

Establishment of a Murine Pulp Exposure Model with a Novel Mouth-Gag for Pulpitis Research

Published on: October 27, 2023

Self-etching increases matrix metalloproteinase expression in the dentin-pulp complex.

N Lehmann¹, R Debret, A Roméas

¹University of Lyon, F-69000, Lyon, France.

Journal of Dental Research

|January 10, 2009

Summary

This summary is machine-generated.

Self-etching adhesives stimulate matrix metalloproteinases (MMPs) in odontoblasts, contributing to hybrid layer degradation in dental restorations. This suggests odontoblasts play a role in the breakdown of the tooth-dentin bond.

More Related Videos

Inducing Apical Periodontitis in Mice

Inducing Apical Periodontitis in Mice

Published on: August 6, 2019

Related Experiment Videos

Last Updated: Jun 26, 2026

Establishment of a Murine Pulp Exposure Model with a Novel Mouth-Gag for Pulpitis Research

Establishment of a Murine Pulp Exposure Model with a Novel Mouth-Gag for Pulpitis Research

Published on: October 27, 2023

Inducing Apical Periodontitis in Mice

Inducing Apical Periodontitis in Mice

Published on: August 6, 2019

Area of Science:

Biomaterials Science
Dental Research
Cell Biology

Background:

Hybrid layer degradation is a significant issue in adhesive dental restorations.
Matrix metalloproteinases (MMPs) in dentin are implicated in this degradation, activated by acidic dental adhesives.

Purpose of the Study:

To investigate if self-etching adhesives stimulate matrix metalloproteinase (MMP) expression in odontoblasts.
To evaluate the role of odontoblasts in hybrid layer degradation following self-etching adhesive application.

Main Methods:

Cultured tooth slices were treated with self-etching adhesive in dentin cavities.
Immunohistochemistry and zymography were used to assess MMP-2 and proMMP-9 expression in the dentin-pulp complex.

Main Results:

Self-etching adhesive treatment increased MMP-2 expression in odontoblasts, confirmed by immunohistochemistry.
Zymography revealed elevated proMMP-9 and MMP-2 levels in dentin when the pulp was present after treatment.

Conclusions:

Self-etching adhesives stimulate MMP secretion from the dentin-pulp complex, particularly by odontoblasts.
Odontoblasts are suggested to actively participate in the degradation of the hybrid layer in adhesive restorations.