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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: Jun 26, 2026

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

A human proteome detection and quantitation project.

N Leigh Anderson1, Norman G Anderson, Terry W Pearson

  • 1The Plasma Proteome Institute, Washington, D. C. 20009, USA. leighanderson@plasmaproteome.org

Molecular & Cellular Proteomics : MCP
|January 10, 2009
PubMed
Summary
This summary is machine-generated.

Developing sensitive, specific, and multiplexable assays for human proteins is crucial for biomarker discovery. Mass spectrometry-based assays offer a systematic approach to quantitatively analyze the human proteome, advancing clinical diagnostics.

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
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Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Published on: March 23, 2020

Related Experiment Videos

Last Updated: Jun 26, 2026

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
11:54

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Published on: March 23, 2020

Area of Science:

  • Biochemistry
  • Proteomics
  • Clinical Diagnostics

Background:

  • The primary obstacle in translating candidate biomarkers into clinical tests is the absence of sensitive, specific, and multiplexable assays for most human proteins.
  • Current assay limitations hinder the comprehensive analysis of the human proteome for diagnostic purposes.

Purpose of the Study:

  • To address the lack of robust protein assays by proposing a systematic and economical approach for comprehensive human proteome quantification.
  • To enable rapid verification of candidate biomarkers and establish a quantitative foundation for further proteomic research.

Main Methods:

  • Leveraging recent advancements in mass spectrometry-based assays for proteotypic peptides.
  • Implementing specific affinity peptide enrichment techniques for enhanced sensitivity and specificity.
  • Proposing the development of a complete suite of assays targeting two peptides per human gene (Proteome Detection and Quantitation project).

Main Results:

  • This approach offers a systematic and economical pathway towards comprehensive quantitative coverage of the human proteome.
  • The proposed assay suite would facilitate rapid and systematic verification of candidate biomarkers.
  • It would establish a quantitative foundation for investigating splice variants, post-translational modifications, protein-protein interactions, and tissue localization.

Conclusions:

  • Mass spectrometry-based assays with affinity enrichment provide a viable solution to the critical need for sensitive and specific protein quantification.
  • The human Proteome Detection and Quantitation project has the potential to revolutionize biomarker discovery and accelerate clinical translation.
  • This quantitative proteomic foundation is essential for future explorations into the complexities of the human proteome.