Intracellular response of caudate neurons to variable frequency stimulation of motor cortical areas in dog

The intracellular response to electrical stimulation of motor cortex was studied in 77 neurons recorded in the head of the caudate (Cd) nucleus of dog. Single pulse stimulation of either medial, intermediate or lateral precruciate cortex produced a response in 69 neurons, 59% of which responded to more than one cortical area. Most intracellular responses were complex potentials consisting of an initial depolarization (E) followed by a longer duration hyperpolarization (I) or E-I response complex. When stimulated with trains of low frequency pulses (10 Hz), the stimulus-generated I potentials reduced the absolute amplitude of the evoked E's, often to a level below resting potential. However, at higher frequencies (50 Hz), the I potentials were attenuated and the E potentials summated into a prolonged depolarization lasting the duration of the stimulus train. A computer model of the response to multiple stimuli was generated assuming that the E-I response to each stimulus pulse in the train should temporally summate with previous responses. As the frequency of stimulation was increased, this model consistently predicted greater summation of the I potentials than was experimentally observed. These data suggest that inhibition of Cd neurons is input frequency dependent such that as the frequency of cortical input increases there is a decrease of input-generated inhibition of Cd neurons. Thus, inhibition may modulate the response of Cd neurons such that cortical input must reach a critical firing frequency before being relayed through the Cd nucleus.