Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phenytoin and retinal spreading depression.

S R Chebabo1, R J Do Carmo

  • 1Instituto de Biofísica Carlos Chagas Filho, Departamento de Neurobiologia, Cidade Universitária-Ilha do Fundão, Rio de Janeiro, Brazil.

Brain Research
|June 14, 1991
PubMed
Summary

Phenytoin, an anticonvulsant, effectively counteracts spreading depression in chick retinas. It increases the stimulus threshold, slows propagation, and shortens the duration of this neurological phenomenon.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The organophosphate sarin, at low concentrations, inhibits the evoked release of GABA in rat hippocampal slices.

Neurotoxicology·2000
Same author

Low concentrations of the organophosphate VX affect spontaneous and evoked transmitter release from hippocampal neurons: toxicological relevance of cholinesterase-independent actions.

Toxicology and applied pharmacology·1999
Same author

Retinal spreading depression induced by photoactivation: involvement of free radicals and potassium.

Brain research·1999
Same author

An analysis of low level doses of cholinesterase inhibitors in cultured neurons and hippocampal slices of rats.

Drug and chemical toxicology·1999
Same author

Hypotonic exposure enhances synaptic transmission and triggers spreading depression in rat hippocampal tissue slices.

Brain research·1995
Same author

Interstitial space, electrical resistance and ion concentrations during hypotonia of rat hippocampal slices.

The Journal of physiology·1995

Area of Science:

  • Neuroscience
  • Pharmacology

Background:

  • Spreading depression is linked to seizures, migraine aura, and neurological conditions like ischemia and trauma.
  • The anticonvulsant phenytoin protects nervous tissue from anoxia and ischemia, suggesting potential efficacy against spreading depression.

Purpose of the Study:

  • To investigate the effects of phenytoin on spreading depression in isolated chick retinas.
  • To determine if phenytoin can modulate the initiation, propagation, and duration of spreading depression.

Main Methods:

  • Spreading depression was elicited using mechanical or chemical (KCl) stimulation in isolated chick retinas.
  • The effects of phenytoin were assessed on the threshold concentration, propagation velocity, and duration of spreading depression.

Main Results:

  • Phenytoin increased the KCl concentration threshold required to initiate spreading depression.
  • Phenytoin decreased the velocity of spreading depression propagation.
  • Phenytoin significantly shortened the duration of slow potential, ionic shifts (K+, Ca2+, Cl-), and extracellular volume changes.

Conclusions:

  • Phenytoin demonstrates significant inhibitory effects on spreading depression in a retinal model.
  • These findings support the potential therapeutic role of phenytoin in conditions associated with spreading depression.

Related Experiment Videos