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Polymerized placenta hemoglobin improves cardiac functional recovery and reduces infarction size of isolated rat
1Laboratory of Anesthesiology and Critical Care Medicine, West China School of Pharmacy, Sichuan University, Chengdu, China.
Objective:
To investigate the cardioprotective effect of polymerized human placenta hemoglobin (PolyPHb) to rat heart subjected to 8-hour hypothermic storage and 2-hour normothermic reperfusion.
Methods And Results:
Isolated rat hearts were perfused with Langendorff model; after 30 minutes of baseline, the hearts were arrested and stored by St. Thomas' solution (STS) without (STS group) or with 0.5 gHb/dL PolyPHb (PolyPHb group) at 4 degrees C for 8 hours, then reperfused for 2 hours. Compared with STS group, PolyPHb in STS greatly improved the recovery of left ventricular developed pressure (LVDP), maximum LVDP increase and decrease rate (+/-dp/dt), coronary flow rate (CF). Also, both the cardiac enzyme release, including creatine kinase (CK) and lactate dehydrogenase (LDH), and myocardial infarction size were significantly reduced in PolyPHb group.
Conclusion:
Our study demonstrated that the PolyPHb was beneficial to improving cardiac functional recovery and reducing myocardial infarction of 8-hour hypothermic stored rat heart.
