Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Replicative Cell Senescence

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...

Cancer Stem Cells and Tumor Maintenance

Cancer Stem Cells and Tumor Maintenance

Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...

Cancer Stem Cells and Tumor Maintenance

Cancer Stem Cells and Tumor Maintenance

Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...

The Retinoblastoma Gene

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Machine Learning-Assisted Development of High-Performance Ethanol Synthesis Catalysts via CO<sub>2</sub> Hydrogenation.

Journal of the American Chemical Society·2026

Same author

The Warburg effect provides attributes that facilitate tumor-cell resistance.

Histology and histopathology·2026

Same author

Peripheral Insulin Resistance and Alzheimer's Disease: Possible Mediators Including Extracellular Vesicles.

Geriatrics & gerontology international·2026

Same author

Machine-Learning-Guided Discovery of CH<sub>4</sub> Combustion Catalysts Operating in the Presence of SO<sub>2</sub>.

Journal of the American Chemical Society·2026

Same author

Reevaluating the senolytic activity of a GLS1 inhibitor and an anti-PD-1 antibody: toward greater reproducibility and methodological rigor.

EMBO reports·2026

Same author

The metabolites for muscle and osteoclast activity are indicators of femoral neck osteoporosis.

Scientific reports·2026

Search research articles

Related Experiment Video

Updated: Jun 26, 2026

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Published on: July 12, 2022

Senescence induction; a possible cancer therapy.

Matilde E Lleonart¹, Ana Artero-Castro, Hiroshi Kondoh

¹Pathology Department, Fundació Institut de Recerca Hospital Vall d'Hebron, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain. melleona@ir.vhebron.net

Molecular Cancer

|January 10, 2009

Summary

Cellular immortalization, a key step in cancer, involves overcoming replicative senescence. Identifying genes like micro-RNAs involved in this process offers new cancer therapy avenues.

More Related Videos

Induction and Validation of Cellular Senescence in Primary Human Cells

Induction and Validation of Cellular Senescence in Primary Human Cells

Published on: June 20, 2018

Related Experiment Videos

Last Updated: Jun 26, 2026

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Published on: July 12, 2022

Induction and Validation of Cellular Senescence in Primary Human Cells

Induction and Validation of Cellular Senescence in Primary Human Cells

Published on: June 20, 2018

Area of Science:

Cell Biology
Cancer Research
Genetics

Background:

Primary mammalian cells undergo replicative senescence, a permanent growth arrest, limiting cellular lifespan.
Senescent cells exhibit altered morphology and gene expression but retain metabolic activity, and are found in human tumors.
Senescence acts as a barrier against cellular immortalization, a critical step in cancer development.

Purpose of the Study:

To investigate the genetic mechanisms underlying cellular senescence and immortalization.
To identify genes, including micro-RNAs, that regulate the transition from senescence to immortality.
To explore new therapeutic strategies for cancer based on modulating senescence.

Main Methods:

Utilized high-throughput genetic screening to identify genes involved in senescence.
Analyzed the roles of tumor suppressor genes and oncogenes in senescence and immortalization.
Examined the expression patterns and functions of candidate genes and micro-RNAs.

Main Results:

Identified several candidate oncogenes and putative tumor suppressor genes that influence senescence.
Confirmed the involvement of tumor suppressor genes and oncogenes in overriding senescence.
Discovered specific micro-RNA subtypes as key players in senescence regulation.

Conclusions:

Cellular immortalization involves overcoming senescence through genetic alterations.
High-throughput screening effectively identified novel genes, including micro-RNAs, implicated in senescence.
Modulating senescence presents promising new therapeutic approaches for human cancer.