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Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
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Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...

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Updated: Jun 26, 2026

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment
12:58

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

Published on: May 25, 2017

Typing Clostridium difficile strains based on tandem repeat sequences.

N Henning Zaiss1, Maja Rupnik, Ed J Kuijper

  • 1Robert Koch Institute, Wernigerode, Germany. zaissn@rki.de

BMC Microbiology
|January 10, 2009
PubMed
Summary
This summary is machine-generated.

New tandem repeat sequencing methods for Clostridium difficile (C. diff) typing offer a portable and effective alternative to PCR ribotyping. This approach accurately tracks epidemic strains and reveals population structures.

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Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
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A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment
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Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
06:51

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Published on: December 10, 2016

Area of Science:

  • Microbiology
  • Genetics
  • Epidemiology

Background:

  • Accurate genotyping of epidemic Clostridium difficile strains is crucial for tracking emergence and spread.
  • Portable genotyping data enhances inter-laboratory comparisons and epidemiological studies.

Purpose of the Study:

  • To identify and characterize novel repetitive DNA loci in C. difficile for sequence-based strain typing.
  • To evaluate the utility of these loci for routine C. difficile genotyping and phylogenetic analysis.

Main Methods:

  • Systematic screening of the C. difficile genome for variation in repetitive DNA.
  • Description and sequencing of two novel tandem repeat loci, TR6 and TR10.
  • Comparison of tandem repeat sequencing with PCR ribotyping using 154 C. difficile isolates.

Main Results:

  • Two tandem repeat loci, TR6 and TR10, exhibiting extensive sequence variation were identified.
  • Tandem repeat sequencing showed excellent concordance and equal discriminatory power compared to PCR ribotyping.
  • Analysis of tandem repeat sequences allowed reconstruction of C. difficile population structure and evolutionary history.

Conclusions:

  • Sequence analysis of TR6 and TR10 provides a highly useful method for routine C. difficile typing.
  • Tandem repeat sequence typing achieves phylogenetic resolution comparable to PCR ribotyping.
  • Digital storage of DNA sequences facilitates data sharing and obviates the need for physical strain exchange.