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Corneal stroma PDGF blockade and myofibroblast development.

Harmeet Kaur1, Shyam S Chaurasia, Fabricio W de Medeiros

  • 1Cole Eye Institute, The Cleveland Clinic, Cleveland, OH 44195, USA.

Experimental Eye Research
|January 10, 2009
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Summary

Blocking platelet-derived growth factor-beta (PDGF-B) in the cornea after photorefractive keratectomy (PRK) reduces myofibroblast development. This finding highlights PDGF-B

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Area of Science:

  • Ophthalmology
  • Corneal Biology
  • Wound Healing

Background:

  • Myofibroblast development and corneal haze after photorefractive keratectomy (PRK) are influenced by cytokines like transforming growth factor-beta (TGF-beta).
  • Platelet-derived growth factor (PDGF) is a potential mediator in corneal stromal responses.

Purpose of the Study:

  • To investigate the effect of blocking stromal platelet-derived growth factor-beta (PDGF-B) on myofibroblast development following PRK in rabbits.
  • To assess the impact of PDGF-B blockade on corneal haze formation post-PRK.

Main Methods:

  • Rabbits underwent photorefractive keratectomy (PRK) for myopia correction.
  • Experimental groups received stromal application of a plasmid targeting PDGF receptor beta (pCMV.PDGFRB.23KDEL), an empty plasmid (pCMV), or balanced salt solution (BSS).
  • Treatment was administered post-surgery for 4-5 days until epithelial healing.

Main Results:

  • Stromal application of pCMV.PDGFRB.23KDEL significantly reduced alphaSMA+ myofibroblast density in the anterior stroma compared to control groups (P
  • A trend towards reduced corneal haze was observed with PDGF-B blockade, but this did not reach statistical significance at one month post-surgery.

Conclusions:

  • Stromal PDGF-B blockade effectively decreases the generation of alphaSMA+ myofibroblasts in the early postoperative period after PRK.
  • PDGF-B plays a significant role in modulating myofibroblast development within the corneal stroma.