Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-Resolution Mass Spectrometry Approach for Proteomic and Metabolomic Analyses of High-Protein Soybean Seeds.

Journal of agricultural and food chemistry·2025
Same author

Protein profiling of fast neutron soybean mutant seeds reveals differential accumulation of seed and iron storage proteins.

Phytochemistry·2022
Same author

Salicylic Acid and Phytoalexin Induction by a Bacterium that Causes Halo Blight in Beans.

Phytopathology·2022
Same author

Bacterial Immobilization and Toxicity Induced by a Bean Plant Immune System.

Journal of proteome research·2021
Same author

Comparative analysis of extracellular proteomes reveals putative effectors of the boxwood blight pathogens, Calonectria henricotiae and C. pseudonaviculata.

Bioscience reports·2021
Same author

Comparative Secretome Analyses of Toxigenic and Atoxigenic <i>Rathayibacter</i> Species.

Phytopathology·2021

Related Experiment Video

Updated: Jun 26, 2026

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
09:13

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

Published on: June 14, 2017

Utility of proteomics techniques for assessing protein expression.

Savithiry S Natarajan1, Chenping Xu, Perry Cregan

  • 1USDA-ARS, Soybean Genomics and Improvement Laboratory, PSI, Beltsville, MD 20705, USA. savi.natarajan@ars.usda.gov

Regulatory Toxicology and Pharmacology : RTP
|January 10, 2009
PubMed
Summary

Natural variation in soybean seed proteins was analyzed using proteomic technologies. Significant differences were found between wild and cultivated soybean genotypes, aiding GMO soybean safety assessments.

More Related Videos

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

Related Experiment Videos

Last Updated: Jun 26, 2026

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
09:13

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

Published on: June 14, 2017

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

Area of Science:

  • Agricultural Science
  • Biochemistry
  • Genetics

Background:

  • Proteomic technologies are essential for analyzing protein profile modifications.
  • Understanding natural soybean seed protein variation is crucial for assessing genetically modified organism (GMO) soybean safety.
  • Evaluating unintended effects of transgenic modifications requires baseline data on natural protein diversity.

Purpose of the Study:

  • To characterize the natural variation in soybean seed protein profiles across different genotypes.
  • To compare protein profiles between wild and cultivated soybean groups.
  • To establish a foundation for comparing genetically modified (GMO) and non-GMO soybeans.

Main Methods:

  • Utilized two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) for protein separation.
  • Employed matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry for protein identification.
  • Applied liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) for protein quantification.

Main Results:

  • Significant variations in allergen and anti-nutritional protein profiles were observed between cultivated and wild soybean genotypes.
  • Minor protein profile variations were detected within the same group (cultivated or wild).
  • Proteomic analysis revealed distinct protein profiles differentiating wild and cultivated soybean groups.

Conclusions:

  • Natural variation in soybean seed proteins is substantial between wild and cultivated types.
  • These findings provide a reference for assessing protein profiles in GMO soybeans.
  • Further research with diverse varieties and locations is recommended for comprehensive GMO safety evaluation.