Cloning and characterization of the nifA gene from Herbaspirillum seropedicae strain Z78
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E M Souza1, S Funayama, L U Rigo
1Department of Biochemistry, Universidade Federal do Parana, Curitibia, PR, Brazil.
Canadian Journal of Microbiology
|June 1, 1991
Abstract:
A genomic library of Herbaspirillum seropedicae was constructed and screened for the nifA gene by complementation of a nifA mutant of Azospirillum brasilense (FP10). A recombinant plasmid, pEMS1, capable of restoring acetylene reduction activity in the mutant FP10, was isolated and found to hybridize to the nifA gene of Klebsiella pneumoniae. The results suggest that nifA is involved in the regulation of nif genes in H. seropedicae.