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Updated: Jun 26, 2026

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Published on: August 31, 2019

Templated spherical high density lipoprotein nanoparticles.

C Shad Thaxton¹, Weston L Daniel, David A Giljohann

¹Northwestern University, Feinberg School of Medicine, Department of Urology, 303 East Chicago Avenue, Tarry 16-703, Chicago, Illinois 60611, USA. cthaxton003@md.northwestern.edu

Journal of the American Chemical Society

|January 13, 2009

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Researchers created artificial high-density lipoprotein (HDL) nanoparticles that mimic natural HDL and effectively bind free cholesterol. This development offers a valuable benchmark for future synthetic HDL research and applications.

Area of Science:

Biomaterials Science
Nanotechnology
Biochemistry

Background:

High-density lipoprotein (HDL) plays a crucial role in reverse cholesterol transport.
Developing synthetic HDL mimics is essential for understanding HDL function and for therapeutic applications.
Current synthetic HDL models often lack the precise size and surface characteristics of natural HDL.

Purpose of the Study:

To synthesize biomimetic high-density lipoprotein (HDL) nanoparticles.
To engineer these nanoparticles for effective free cholesterol binding.
To establish a quantitative measure for evaluating synthetic HDL performance.

Main Methods:

Utilizing a gold nanoparticle core to template nanoparticle assembly.
Forming a mixed phospholipid layer around the core.

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Last Updated: Jun 26, 2026

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Published on: August 31, 2019

Formulation and Characterization of Bioactive Agent Containing Nanodisks

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Preparation and Characterization of Novel HDL-mimicking Nanoparticles for Nerve Growth Factor Encapsulation

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Published on: May 22, 2017

Adsorbing apolipoprotein A-I to create a biomimetic surface.

Quantifying cholesterol binding affinity using K(d) determination.

Main Results:

Successfully synthesized HDL biomimetic nanoparticles with natural HDL size and surface composition.
Demonstrated the ability of these nanoparticles to bind free cholesterol.
Determined a high binding affinity for free cholesterol (K(d) = 4 nM).

Conclusions:

The synthesized HDL biomimetic nanoparticles effectively mimic natural HDL structure and function.
These nanoparticles represent a significant advancement in the development of synthetic HDL.
The quantified cholesterol binding affinity provides a critical benchmark for future research and development.