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Updated: Jun 26, 2026

Single-Copy Gene Locus Chromatin Purification in Saccharomyces cerevisiae
Published on: November 17, 2023
Construction and characterization of single-gene chromosomes in Saccharomyces cerevisiae
Kazuo Yamagishi1, Minetaka Sugiyama, Yoshinobu Kaneko
1Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita-shi, Osaka 565-0871, Japan.
Abstract:
Based on a previously developed PCR-mediated chromosome splitting method, a genome engineering technique was developed in haploid Saccharomyces cerevisiae for incorporating any desired chromosomal region into a chromosome that carries a single gene. Based on the viability of cells carrying an essential gene in such a construct, close physical proximity of two telomeres and a centromere does not appear to compromise gene function. Spontaneous loss of constructed single-gene chromosomes during vegetative growth was high (0.2-0.4 per cell division), suggesting the possibility of creating novel cells carrying single-gene chromosomes derived from various chromosomal regions in a variety of combinations by exploiting combinatorial loss.
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