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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Related Experiment Video

Updated: Jun 26, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Visual, base-specific detection of nucleic acid hybridization using polymerization-based amplification.

Ryan R Hansen1, Leah M Johnson, Christopher N Bowman

  • 1Department of Chemical and Biological Engineering, University of Colorado, ECCH 111 CB 424, Boulder, CO 80309, USA.

Analytical Biochemistry
|January 13, 2009
PubMed
Summary
This summary is machine-generated.

This study introduces a novel polymerization method for sensitive, visual detection of DNA. The technique enables accurate point mutation and single nucleotide polymorphism (SNP) typing, even in complex samples.

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ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
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ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

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Visual Detection of Multiple Nucleic Acids in a Capillary Array
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Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection
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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Diagnostics

Background:

  • Biotinylated biomolecules on microarrays can be visualized using polymerization-based signal amplification for point-of-care applications.
  • Sensitive detection of nucleic acid hybridizations is crucial for various diagnostic applications.

Purpose of the Study:

  • To adapt polymerization-based signal amplification for visual detection of multiplexed nucleic acid hybridizations.
  • To develop a method for point mutation detection and single nucleotide polymorphism (SNP) typing.

Main Methods:

  • Utilized primer extension reactions to label DNA hybrids with photoinitiators.
  • Employed simultaneous and dynamic photopolymerization for signal amplification.
  • Applied the method to detect nucleic acid hybridizations in complex media.

Main Results:

  • Achieved sensitive detection of target concentrations down to 0.5 nM.
  • Demonstrated visual discrimination of single base differences in KRAS codon-12 biomarkers.
  • Significantly improved signal ratios between complementary and mismatched hybrids.

Conclusions:

  • Polymerization-based signal amplification is effective for sensitive, visual detection of nucleic acid hybridizations.
  • The developed method allows for accurate point mutation and SNP typing.
  • This approach is suitable for point-of-care diagnostics.