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Related Concept Videos

Adrenergic Neurons: Neurotransmission01:27

Adrenergic Neurons: Neurotransmission

Postganglionic sympathetic fibers (except those supplying the sweat glands) releasing noradrenaline or norepinephrine are called noradrenergic or adrenergic neurons. Noradrenaline, dopamine, adrenaline, or epinephrine are collectively called "catecholamines" as they contain a catechol moiety and an amine side chain. The five stages of neurotransmitter release involve their synthesis, storage, release, reuptake and metabolism.
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Related Experiment Video

Updated: Jun 26, 2026

Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs
09:37

Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs

Published on: August 31, 2009

Neuronal release of proBDNF.

Jianmin Yang1, Chia-Jen Siao, Guhan Nagappan

  • 1Department of Medicine, Weill Cornell Medical College, 1300 York Ave., New York, New York 10065, USA.

Nature Neuroscience
|January 13, 2009
PubMed
Summary

Neurons secrete both pro-brain-derived neurotrophic factor (proBDNF) and mature BDNF, with levels changing during development. These neurotrophins and their receptors, p75 and TrkB, regulate neuronal actions differently across lifespan.

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Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • Pro-brain-derived neurotrophic factor (proBDNF) and mature BDNF are distinct isoforms of BDNF.
  • These isoforms bind to different receptors, mediating divergent neuronal functions.
  • Understanding the dynamic expression of these isoforms is crucial for comprehending neuronal development and function.

Purpose of the Study:

  • To quantify endogenous BDNF isoforms (proBDNF and mature BDNF) secreted by mouse neurons.
  • To investigate the developmental regulation of BDNF isoforms and their receptors (p75 and TrkB).
  • To elucidate how differential secretion and receptor expression influence neuronal actions throughout development.

Main Methods:

  • Utilized novel tools for precise quantification of endogenous BDNF isoforms.
  • Analyzed BDNF isoform and receptor expression in mouse neurons across different developmental stages (perinatal to adult).

Main Results:

  • Mouse neurons were found to secrete both proBDNF and mature BDNF.
  • The highest levels of proBDNF and the p75 receptor were observed during the perinatal period, decreasing but remaining detectable in adulthood.
  • BDNF actions are modulated by the developmental stage and the specific BDNF isoform secreted.

Conclusions:

  • BDNF signaling is developmentally regulated, influenced by the balance of secreted proBDNF and mature BDNF.
  • The expression levels of p75 and TrkB receptors further contribute to the differential actions of BDNF isoforms.
  • This study provides new insights into the complex mechanisms governing neurotrophic factor actions during neuronal development.