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MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
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miRNA in situ hybridization in formaldehyde and EDC-fixed tissues

John T G Pena1, Cherin Sohn-Lee, Sara H Rouhanifard

  • 1Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10065, USA.

Nature Methods
|January 13, 2009
PubMed

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In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...