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Related Experiment Video

Updated: Jun 26, 2026

Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

Hyphenated tools for phospholipidomics.

Jan Willmann1, Dieter Leibfritz, Herbert Thiele

  • 1Department of Organic Chemistry, University of Bremen, Bremen, Germany.

Journal of Biomolecular Techniques : JBT
|January 13, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Proteomics. Clinical applications·2020

Reliable analysis of intact lipids in biological samples is crucial for early diagnosis. This study presents an automated workflow combining multiple techniques for accurate lipid detection and characterization.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Clinical Diagnostics

Background:

  • Accurate lipid analysis is vital for early disease diagnosis.
  • Current methods require fast, reliable, and automated analytical workflows.
  • Detecting known and unknown lipid species presents a significant challenge.

Purpose of the Study:

  • To present a workflow for the reliable analysis of individual components in phosphatidylethanolamine extracts.
  • To evaluate the limitations and advantages of individual analytical methods.
  • To enable early diagnosis through advanced lipidomic analysis.

Main Methods:

  • Solid phase extraction (SPE) for sample preparation.
  • High-performance liquid chromatography (HPLC) for separation.

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Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics Analysis
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Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics Analysis

Published on: October 27, 2023

Related Experiment Videos

Last Updated: Jun 26, 2026

Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics Analysis
09:28

Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics Analysis

Published on: October 27, 2023

  • Mass spectrometry (MS) for detection and identification.
  • Nuclear magnetic resonance (NMR) spectroscopy for structural elucidation.

    • Main Results:

    • Demonstration of a workflow for reliable analysis of phosphatidylethanolamine components.
    • Discussion of the strengths and weaknesses of each analytical technique.
    • Potential for improved detection of lipid biomarkers.

    Conclusions:

    • The integrated workflow offers a robust approach for lipidomic analysis.
    • This method supports the accurate identification of lipid species in complex biological matrices.
    • Advancements in analytical techniques are key to improving early diagnostic capabilities.