Effects of adenine on the pituitary-gonad axis in newborns rats

The present study was undertaken to investigate effects of the adenine on the hypothalamus-pituitary-gonad axis and changes in blood hormone concentration such as FSH, LH, progesterone and estrogen in newborn female rats. Adenine is a common organic base and its concentration variations caused by foods, has various effects on the body metabolic systems. In present study, fifty newborns rats were used divided into five groups, of 10s, including control I, control II which received solvent (normal saline) only and three experimental groups which received 50, 100 and 200 mg body weight adenine respectively. All the animals were kept under same condition with plenty food and water and treated Intra Peritoneally (IP) during days 2-16 after birth. At the end of experiment, all the animals were weighed, their ovaries were removed and blood samples were taken for hormone analysis. The results showed that dose dependent adenine solution significantly reduced the body and ovarian weight on 30 and 70 days after birth. In addition adenine led into no significant difference in concentration of FSH and LH in the experimental groups relative to the control on 30th day of life. But on the 70th day, the levels of these hormones raised significantly in the experimental groups. Furthermore, the adenine solution significantly increased the levels of progesterone and estrogen hormones in the experimental groups relative to the control on the 30th day, while decreased their concentration significantly on the 70th day. This situation has close similarities to metabolic disorders present in human caused by excessive use of adenine. High amounts consumption of adenine in can lead into hormone abnormality, weight loss and metabolic anomalies.