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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

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Related Experiment Video

Updated: Jun 26, 2026

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points
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Published on: May 29, 2020

[Silencing LRP1B with huge transcript by RNAi method].

Ruo Li1, Yan-Yan Tian, Yong Gao

  • 1Department of Life Science and Bioengineering, Harbin Institute of Technology, Harbin 150001, China. liruo1999@163.com

Yi Chuan = Hereditas
|January 14, 2009
PubMed
Summary

Designing effective RNAi requires considering mRNA secondary structure, especially for large genes like Lipoprotein receptor-related protein 1 B (LRP1B). This study developed a method to efficiently silence LRP1B gene expression for further research.

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Published on: May 30, 2025

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Context:

  • Lipoprotein receptor-related protein 1 B (LRP1B) is a large receptor involved in cellular processes.
  • Studying LRP1B function is challenging due to its massive 16.5 kb transcript.
  • The role of LRP1B in tumor metastasis requires effective gene silencing methods.

Purpose:

  • To develop and validate an efficient siRNA design strategy for targeting large gene transcripts.
  • To investigate the relationship between LRP1B expression and tumor metastasis by silencing the gene.
  • To assess the efficacy of RNA interference (RNAi) in suppressing LRP1B expression.

Summary:

  • A novel siRNA design method was employed, integrating mRNA secondary structure analysis to select optimal target sites for the large LRP1B transcript.
  • Six candidate siRNAs were designed and tested, with five demonstrating effective silencing (>50%) of LRP1B expression in HEK 293 cells.
  • Monoclonal cells with near-complete LRP1B silencing were successfully generated, validating the approach.

Impact:

  • The developed siRNA design strategy offers a feasible and efficient method for targeting large and complex gene transcripts.
  • This approach facilitates functional studies of genes like LRP1B, previously hindered by transcript size.
  • The successful silencing of LRP1B provides a tool for further investigating its role in tumor metastasis.