Related Experiment Video
Updated: Jun 26, 2026

Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis
Published on: February 13, 2014
[NF-kappaB modulates activation of the BMP-2 gene by trichostatin A]
Abstract:
In this study, we investigated trichostatin A (TSA), a histone deacetylase inhibitor, increased the Bone morphogenetic protein-2 (BMP-2) mRNA level in human osteoblasts line. Deletion analysis of the promoter region revealed that TSA-induced luciferase was regulated by the BMP-2 promoter spanning from -320 to-310. Electrophoresis mobility shift assay (EMSA) and Chromatin immunoprecipitation (CHIP) assay proved that this position was nuclear factor NF-kappaB responsive element. The results above demonstrated that acetylation plays a crucial role in BMP-2 expression and acetylation of NF-kappaB p65 and p50 subunits by TSA treatment may activate the BMP-2 promoter.
Related Concept Videos
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Regulation of Nuclear Protein Sorting
NF-kB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
TGF - β Signaling Pathway
Co-activators and Co-repressors
Canonical Wnt Signaling Pathway

