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Updated: Jun 26, 2026

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
[Chemical characteristic of structure components of Pragia fontium lipopolysaccharides]
Abstract:
Structure components (lipid A. oligosaccharide core, and O-specific polysaccharide) of the lipopolysaccharide from 8 strains of Pragia fontium were obtained as a result of mild acidic hydrolysis. The macromolecular organization of lipopolysaccharides of studied strains was heterogeneous and presented both by S-forms, and R- or SR-forms. It was established that as to fatty acid composition lipids A of tested strains were represented by one chemotype with predominant 3-hydroxytetradecanoic fatty acid. An analysis ofmonosaccharide composition of core oligosaccharides has detected 5 chemotypes, and glucose, galactose, rhamnose, ribose, arabinose, xylose and mannose were identified in them. It was shown that O-specific polysaccharides consisted of glucose, galactose, rhamnose, and mannose that demonstrated their belonging to 4 chemotypes.
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