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Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution
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Cryopreserved tissue engineered mucosa.

Gabriele Spoerl1, Ronald Mai, Edith Klingner

  • 1Institute for Air Conditioning and Refrigeration, Department of Applied New Technologies, Dresden, Germany.

Folia Histochemica Et Cytobiologica
|January 15, 2009
PubMed
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Established cryopreservation protocols exist for single cells but not for tissue engineered grafts. This study developed a cryopreservation protocol and a nude rat model to assess the efficacy of cryopreserved engineered mucosa grafts.

Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Clinical application of grafts relies on cryopreservation, with established protocols for single cell suspensions.
  • Tissue engineered constructs, increasingly used as grafts, lack standardized cryopreservation protocols and viability assays.
  • Assessing graft function post-cryopreservation remains a challenge for tissue engineered products.

Purpose of the Study:

  • To develop a cryopreservation protocol for tissue engineered mucosa grafts.
  • To establish an animal model for testing the efficacy of cryopreserved engineered grafts.
  • To evaluate the viability and function of cryopreserved engineered mucosa in vivo.

Main Methods:

  • Tissue engineered mucosa grafts, comprising keratinocytes and fibroblasts in a collagen sponge, were cryopreserved.

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Tissue Engineering of the Intestine in a Murine Model
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Tissue Engineering of the Intestine in a Murine Model

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Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution
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Tissue Engineering of the Intestine in a Murine Model
08:45

Tissue Engineering of the Intestine in a Murine Model

Published on: December 1, 2012

  • Grafts were implanted into nude rats to assess their efficacy as functional mucosa grafts.
  • Graft healing was evaluated at one and three weeks post-implantation.

    • Main Results:

    • Cryopreservation allowed sufficient cell survival for epithelial-fibroblast tissue development within the collagen sponge.
    • After three weeks, mucosa tissue formation was more complete, with significant degradation of the collagen scaffold.
    • The nude rat model demonstrated suitability for assessing cryopreserved engineered mucosa graft efficacy.

    Conclusions:

    • The developed cryopreservation protocol supports the viability of engineered mucosa grafts.
    • The nude rat model serves as a viable platform for evaluating cryopreserved tissue engineered grafts.
    • In vivo formation of human epithelial-fibroblast tissue confirms the principle of cryopreserving tissue engineered grafts.