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Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

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Published on: July 14, 2015

Polymer-based monolithic columns in capillary format tailored by using controlled in situ polymerization.

Hiroshi Aoki¹, Nobuo Tanaka, Takuya Kubo

¹Division of Applied Science for Functionality, Graduate School of Science & Technology, Kyoto Institute of Technology, Kyoto, Japan. hjn62658@mtb.biglobe.ne.jp

Journal of Separation Science

|January 15, 2009

Summary

New polymer monolithic columns offer high performance for analyzing drug candidates in miniaturized liquid chromatography (LC) systems. These capillary columns feature a unique bicontinuous structure for enhanced efficiency and faster analysis times.

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Area of Science:

Polymer Chemistry
Analytical Chemistry
Separation Science

Background:

Polymer-based monolithic columns offer an alternative to traditional silica monoliths for liquid chromatography (LC).
Miniaturization of LC systems is crucial for achieving higher throughput and shorter analytical times.
A fine, permeable bicontinuous monolithic structure with submicron domain sizes is key for high-performance LC.

Purpose of the Study:

To introduce novel polymer-based monolithic columns with high performance for small solute analysis, such as drug candidates.
To illustrate the fabrication of these capillary LC columns.
To compare the performance of polymer monoliths with existing silica monoliths.

Main Methods:

Fabrication of polymer-based monolithic columns in capillary format using free radical polymerization (glycerin 1,3-dimethacrylate, GDMA) and stepwise polymerization (epoxy and diamino monomers).
Utilized specially designed porogenic solvents to control monolithic morphology via reaction-induced phase separation.
Evaluated column performance using micro-high-performance liquid chromatography (micro-HPLC) and discussed data with a prototyped wired chip device.

Main Results:

Both GDMA- and epoxy-based monolithic columns in capillary format exhibited a fine bicontinuous structure.
The columns demonstrated a good balance between efficiency (H) and permeability (D).
The epoxy-based column showed excellent separation impedance (E = H(2)/D), indicating superior performance.

Conclusions:

Polymer-based monolithic columns, particularly the epoxy-based ones, offer a promising high-performance alternative for miniaturized LC systems.
The developed fabrication method, using controlled porogenic solvents, effectively creates monolithic structures suitable for efficient small solute separation.
These columns are well-suited for applications requiring high throughput and rapid analysis, such as drug candidate screening.