Molluscicidal saponins from leaves of Hedera canariensis

Activity-guided fractionation of the methanol extract of Hedera canariensis (var. Gloire de Maringo) Wild leaves afforded five saponins 1-5. Chemical and spectral methods (MS, 1HNMR, 13CNMR) showed that they are glycosides of hederagenin and oleanolic acid. The results showed that 4,5 exhibited molluscicidal properties, compound 1 was inactive. Mortality rate of exposed snails increased by increasing plant extract concentration. Lymnaea cailliaudi was more sensitive to plant extract than Biomphalaria alexandrina. The histopathological study revealed distinct damage in the structure of the stomach and ovotestis of treated L. cailliaudi snails. Saponin content was determined in term of haemolytic index.