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Updated: Jun 26, 2026
Microbiological Rapid On-Site Evaluation for Pulmonary Infectious Diseases
Published on: March 1, 2024
[Microbiological characterization of pneumonia in patients of intensive care unit]
Alicja Budak1, Dorota Włodarczyk, Małgorzata Skałkowska
1Zakład Mikrobiologii Farmaceutycznej Uniwersytet Jagielloński Collegium Medium.
Abstract:
The aim of study was bacteria characterization, isolated from 30 patients with microbiologically confirmed pneumonia, hospitalized in ICU of Rydygier's Hospital in Krakow. In the study, endotracheal aspirates (ETA) were being collected and quantitatively inoculated. Bacteria isolation at the concentration of > or =10(6) CFU/ml was recognized as bacteriological etiology of pneumonia. Studied 140 clinical materials and isolated 251 bacterial strains from 16 divers species. Isolation of bacteria at the concentration of > or = 10(6) CFU/ml, on the day of clinically recognized HAP, was observed in 26 patients and presence of 1 species of bacteria at the pathological concentration was detected in 17 patients. In the study of endotracheal aspirates, which were collected after 3 and 6 days of treatment, isolation of bacteria species at the significant concentration was observed appropriate in 15 and 10 patients. In the etiology of infections predominated: P. aeruginosa, A. baumannii, S. maltophilia (17 strains; 47.2%) and Enterobacteriaceae bacilli (12 strains; 33%), including bacilli ESBL-positive. The surveillance of bacterial susceptibility patterns detected high percentage of resistance P. aeruginosa strains to imipenem (38.7%).
Insights
This study characterized bacteria in 30 intensive care unit pneumonia patients, identifying common pathogens like Pseudomonas aeruginosa and Acinetobacter baumannii. High resistance to imipenem was noted in P. aeruginosa strains.
Area of Science:
- Microbiology
- Infectious Diseases
- Critical Care Medicine
Context:
- Pneumonia is a significant cause of mortality in intensive care units (ICUs).
- Bacterial characterization is crucial for understanding hospital-acquired pneumonia (HAP) etiology.
- This study focused on patients hospitalized in the ICU of Rydygier's Hospital in Krakow.
Purpose:
- To characterize bacterial strains isolated from patients with microbiologically confirmed pneumonia.
- To determine the bacteriological etiology of pneumonia in ICU patients.
- To assess bacterial susceptibility patterns, particularly resistance to imipenem.
Summary:
- Endotracheal aspirates (ETA) from 30 ICU pneumonia patients were analyzed, yielding 251 bacterial strains from 16 species.
- Pseudomonas aeruginosa, Acinetobacter baumannii, and S. maltophilia predominated (47.2%), followed by Enterobacteriaceae (33%), including ESBL-positive strains.
- Significant bacterial concentrations (> or = 10(6) CFU/ml) were detected in 26 patients upon HAP diagnosis, with decreasing prevalence during treatment.
Impact:
- Identifies key bacterial pathogens contributing to HAP in the studied ICU population.
- Highlights the prevalence of multidrug-resistant organisms, such as ESBL-producing Enterobacteriaceae.
- Reveals a high resistance rate of P. aeruginosa to imipenem, informing empirical antibiotic strategies.
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