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Updated: Jun 26, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
Published on: August 26, 2012
Molecular mechanisms of archaeal RNA polymerase
Dina Grohmann1, Angela Hirtreiter, Finn Werner
1UCL Institute for Structural and Molecular Biology, Division of Biosciences, Darwin Building, Gower Street, London WC1E 6BT, UK.
Abstract:
All cellular life depends on multisubunit RNAPs (RNA polymerases) that are evolutionarily related through the three domains of life. Archaeal RNAPs encompass 12 subunits that contribute in different ways to the assembly and stability of the enzyme, nucleic acid binding, catalysis and specific regulatory interactions with transcription factors. The recent development of methods to reconstitute archaeal RNAP from recombinant materials in conjunction with structural information of multisubunit RNAPs present a potent opportunity to investigate the molecular mechanisms of transcription.
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