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Updated: Jun 26, 2026

Adaptation at the Extremes of Life: Experimental Evolution with the Extremophile Archaeon Sulfolobus acidocaldarius
Published on: June 14, 2024
Genetic analyses in the hyperthermophilic archaeon Sulfolobus islandicus
Qunxin She1, Changyi Zhang, Ling Deng
1Danish Archaea Centre, Department of Biology, University of Copenhagen, Biocenter, Ole Maaloes Vej 5, DK-2200 Copenhagen N, Denmark. qunxin@bio.ku.dk
Abstract:
Sulfolobus belongs to the hyperthermophilic archaea and it serves as a model organism to study archaeal molecular biology and evolution. In the last few years, we have focused on developing genetic systems for Sulfolobus islandicus using pyrEF as a selection marker and versatile genetic tools have been developed, including methods for constructing gene knockouts and for identifying essential genes. These genetic tools enable us to conduct genetic analysis on the functions of the genes involved in DNA replication and repair processes in S. islandicus and they should also facilitate in vivo analysis of functions of other genes in this model organism.
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