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Related Concept Videos

Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...

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Related Experiment Video

Updated: Jun 26, 2026

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

Replication forks, chromatin loops and dormant replication origins.

J Julian Blow1, Xin Quan Ge

  • 1Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK. j.j.blow@dundee.ac.uk

Genome Biology
|January 16, 2009
PubMed
Summary

Cells activate dormant DNA replication origins when replication slows. This adaptation involves reorganizing chromatin loops and maintaining new origin usage patterns in future cell cycles.

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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Related Experiment Videos

Last Updated: Jun 26, 2026

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • DNA replication is a fundamental process for cell division.
  • Replication origins are specific DNA sites where replication initiates.
  • Under stress, dormant origins can be activated to ensure complete genome duplication.

Purpose of the Study:

  • To investigate cellular adaptation mechanisms to slowed DNA replication.
  • To understand how cells reorganize chromatin and origin usage.
  • To determine if altered origin usage patterns are maintained across cell cycles.

Main Methods:

  • Utilized techniques to monitor DNA replication timing and origin firing.
  • Employed chromatin conformation capture methods to analyze loop organization.
  • Performed long-term cell culture to observe inheritance of origin usage patterns.

Main Results:

  • Slowed DNA replication triggers the activation of previously dormant replication origins.
  • Cells dynamically alter chromatin loop organization in response to replication stress.
  • The newly established pattern of replication origin usage is stably maintained through successive cell divisions.

Conclusions:

  • Cellular adaptation to replication stress involves significant chromatin remodeling.
  • Stable inheritance of altered replication origin programs ensures genomic integrity.
  • This study reveals a novel layer of epigenetic memory in DNA replication.